Bacteria Protein Extraction Buffer has been designed for fast and easy extraction of total proteins from bacteria without sonication or precipitation processes. The buffer utilizes a proprietary non-ionic detergent and is effective in extracting proteins from gram positive and gram negative bacteria. In addition, it also offers several-fold increases in the yield of soluble proteins and the extracted proteins are highly stable, since there is no repeated freezing and thawing steps. Our Bacterial Protein Extraction Buffer is an optimal buffer solution for routinely extracting recombinant proteins from bacterial samples.